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Abbexa’s One-Step RT-qPCR Kit (SYBR) provides high sensitivity of the target RNA level due to its reverse transcriptase and RNase inhibitors mix which aim to diminish RNA degradation and mispriming during reaction setup and reverse transcription to guarantee optimal RT efficiency. The kit also contains SYBR Green Master mix, 2X concentrated, ready for use Master Mix reaction enhanced for dye-based quantitative PCR (qPCR) and compatible with the majority of commercially available real-time PCR systems (ROX-independent and ROX-dependent). The Master mix contains hot-start Taq DNA polymerase, dNTPs, MgCl2, SYBR Green I dye, enhancers, stabilizers and essentials for a success PCR reaction.
| Target | One-Step RT-qPCR Kit (SYBR) |
| Tested Applications | RT-PCR |
| Storage | Store at 4 °C for up to 3 months. For long-term storage, store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Buffer | Contains 30-50 mM Tris-HCl, 0.3-0.5 mg/ml BSA and < 0.3% Triton X-100. |
| Availability | Shipped within 10-15 working days. |
| Note | This product is for research use only. |
